FastQCFastQC Report
Wed 4 Feb 2015
SE363_CAGATC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE363_CAGATC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13972997
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC897790.6425178506801368TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG112200.038.5164611
ACACGTC113000.038.2837313
CGTCTGA112400.038.2668316
TCGGAAG114250.038.1208423
ACGTCTG114200.037.8223515
CACGTCT114850.037.66705314
CGTATGC112450.037.4298344
GATCGGA115400.037.4188461
GAACTCC114950.037.3232921
CGGAAGA117550.037.0116044
CCAGATC113100.036.98891433
TCGTATG114000.036.96053343
AGTCACC113800.036.9541128
CTCGTAT114800.036.7995542
ATCTCGT115550.036.6912640
CATCTCG115300.036.6706139
GTATGCC116400.036.29501345
TCTCGTA116900.036.21367641
CACACGT120700.035.8413112
AGAGCAC122500.035.424218
Produced by FastQC (version 0.11.2)