FastQCFastQC Report
Wed 4 Feb 2015
SE362_GCCAAT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE362_GCCAAT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30157356
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1752410.5810887400075789TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC214800.039.3053730
GTCACGC220950.038.38312529
CACGCCA225200.037.47009731
CACGTCT233100.037.4268914
GATCGGA235150.037.3780441
ACACGTC233750.037.3033813
GCACACG235800.037.2843111
CGTCTGA234650.037.15028816
ACGTCTG235500.037.0360415
CGTATGC232400.036.9864644
AGTCACG230300.036.9692128
TCGGAAG240700.036.9463123
CGGAAGA241250.036.786814
GAACTCC233750.036.7466421
CGCCAAT230000.036.70075233
TCGTATG237650.036.18832443
ACGCCAA234350.036.1327732
CTCGTAT239450.035.8295542
TATCTCG239850.035.74486539
GTATGCC244000.035.2280945
Produced by FastQC (version 0.11.2)