FastQCFastQC Report
Wed 4 Feb 2015
SE361_ACAGTG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE361_ACAGTG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15488601
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2127711.3737263940106663TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG260350.041.41592415
GTATGCC257900.041.38600545
CGTATGC258700.041.26672744
ACACGTC262050.041.2328313
GCACACG264450.041.0626611
CACGTCT263200.040.9245914
CTCGTAT262100.040.60960842
CACACGT267400.040.6012312
CGTCTGA266150.040.4617516
TCGTATG266050.040.15218743
GTCACAC263450.040.0573229
ACTCCAG267600.039.95188523
CAGTCAC265250.039.94305827
GAACTCC269700.039.79044321
GATCTCG267800.039.73562239
GATCGGA276150.039.7065281
CCAGTCA267800.039.5190526
CACAGTG266950.039.51807433
AGCACAC276050.039.45913310
TCCAGTC269300.039.40791725
Produced by FastQC (version 0.11.2)