FastQCFastQC Report
Wed 4 Feb 2015
SE360_TGACCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE360_TGACCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17936999
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4796682.6741820078152427TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG550100.043.05160515
GCACACG553700.042.90936311
ACACGTC552900.042.86158813
CACACGT556750.042.6581312
CACGTCT555250.042.65205814
GTATGCC546350.042.5314845
CGTATGC546750.042.45919844
CGTCTGA557850.042.3797316
GATCGGA571950.041.9616431
ACTGACC554450.041.8383932
CAGTCAC560900.041.8107727
CTCGTAT556050.041.76743742
TCGTATG558300.041.74218843
AGCACAC570450.041.70428510
CTGACCA555400.041.63023833
ACTCCAG562550.041.61289223
GAACTCC564100.041.59756521
CCAGTCA563350.041.53452326
GTCACTG567350.041.45333529
TCCAGTC565500.041.3963925
Produced by FastQC (version 0.11.2)