FastQCFastQC Report
Wed 4 Feb 2015
SE359_TTAGGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE359_TTAGGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8621359
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1629791.8904096210353845TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG176650.043.2985831
ACTTAGG176550.043.2966132
GTATGCC179750.042.5634545
GCACACG182900.042.47105411
CGTATGC180500.042.28683544
CACGTCT183400.042.2816714
ACACGTC183500.042.2708913
ACGTCTG184000.042.24160815
AGGCATC178200.042.21382536
CTCGTAT181500.042.01298542
CAGTCAC184250.041.83579327
CACACGT185600.041.8289712
GGCATCT179750.041.8230837
TCGTATG183450.041.71750343
CCAGTCA184450.041.70358726
GATCGGA185950.041.599171
CGTCTGA187300.041.4966416
TTAGGCA182700.041.44624734
ACTCCAG186300.041.42230623
AGCACAC189650.041.01873810
Produced by FastQC (version 0.11.2)