FastQCFastQC Report
Wed 4 Feb 2015
SE358_CGATGT_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE358_CGATGT_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11501398
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4904824.264542449535265TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC551850.043.48515713
CACACGT552350.043.4824512
GTATGCC544350.043.4331245
CACGTCT553200.043.3627714
GCACACG554450.043.34210611
ACGTCTG554250.043.30903615
TCGTATG549350.043.12384843
CTCGTAT548600.043.06473542
CGTATGC549050.043.03672444
CGTCTGA557900.042.97655516
AGCACAC562100.042.77205710
AGTCACC555950.042.7457128
CGATGTA553450.042.65109334
TCGGAAG570250.042.615183
GATCGGA568750.042.610121
CAGTCAC559600.042.48663327
GAACTCC561600.042.44344721
TCTCGTA557000.042.42611741
CCGATGT557800.042.38358333
CCAGTCA560150.042.37751826