FastQCFastQC Report
Wed 4 Feb 2015
SE357_ATCACG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE357_ATCACG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11197542
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC2254192.013111448923344TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT260200.042.28709814
ACACGTC261100.042.21850613
CGTCTGA260950.042.1044616
TCGGAAG264800.042.08683
GCACACG262850.041.963111
GATCGGA264250.041.96181
ACGTCTG262100.041.93782815
CTCGTAT259600.041.66851842
CGGAAGA267350.041.6515244
TCGTATG261400.041.53954343
CACACGT265600.041.52861812
CGTATGC261250.041.5030844
GAACTCC263050.041.45430821
TCTCGTA261250.041.3780241
AGAGCAC268900.041.352878
GTATGCC263150.041.2547445
AGCACAC267900.041.24765810
ACGATCT260650.041.22173737
ATCGGAA270250.041.1346132
GAGCACA270400.041.0661549
Produced by FastQC (version 0.11.2)