FastQCFastQC Report
Wed 4 Feb 2015
SE356_GGTAGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE356_GGTAGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8650478
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC6230697.202711803902629TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG708600.044.2345543
GATCGGA706550.044.1682431
CACGTCT705850.044.0053114
GCACACG706600.043.9999911
ACACGTC706250.043.99949313
CGGAAGA712300.043.9726874
ACGTCTG708400.043.85668615
ATCGGAA712650.043.8364562
GAGCACA711300.043.8199469
CGTCTGA703050.043.8150216
AGAGCAC713350.043.7941788
CACACGT710650.043.73340212
CACGGTA695200.043.66149531
AGCACAC713100.043.65255410
GTCACGG704900.043.62957429
GAACTCC701800.043.60067421
CGGTAGC690350.043.53597333
ACGGTAG697750.043.5175532
GAAGAGC719900.043.4832156
TCACGGT698650.043.45952630
Produced by FastQC (version 0.11.2)