FastQCFastQC Report
Wed 4 Feb 2015
SE355_GAGTGG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE355_GAGTGG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8034675
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC1893732.356946609539278TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA217050.043.071271
TCGGAAG217950.042.9793663
GCACACG218100.042.52638611
CGGAAGA219950.042.5059434
ACACGTC218350.042.45708513
CACGTCT218350.042.4264414
CGTCTGA218250.042.36289616
CGAGTGG212600.042.28951333
CACGAGT213550.042.27764531
ACGTCTG219250.042.23176615
ACGAGTG214300.042.18245332
AGTCACG217300.042.01605228
ATCGGAA222950.041.978262
GTCACGA217850.041.92526229
GAACTCC220050.041.8957821
TCACGAG217650.041.89376430
AGAGCAC222000.041.8395778
GGATCTC215850.041.65826838
GTGGATC214300.041.59517336
CACACGT223200.041.58491512