FastQCFastQC Report
Wed 4 Feb 2015
SE354_GTGAAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE354_GTGAAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007255
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30550.019.7351911
ACGTCTG30300.019.52693715
AGTCACG26350.019.0415428
CACGTCT30600.019.0413514
ACACGTC31600.018.93704613
CACACGT31800.018.67645812
GTCACGT27050.018.1334529
CGTCTGA34550.017.1897116
CTCGTAT29450.015.51483644
AGCACAC39450.015.45396810
CAGTCAC33350.015.38184627
CCAGTCA35300.014.85042926
CGATCTC30850.014.80751440
ACTCCAG38450.014.8038723
GATCTCG30950.014.68848641
TCGTATG32000.014.63018445
GATCGGA41150.014.499661
GAACTCC39550.014.39146821
TCCAGTC37800.014.1658625
ACGATCT32250.014.09427939
Produced by FastQC (version 0.11.2)