FastQCFastQC Report
Wed 4 Feb 2015
SE353_GTCCGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE353_GTCCGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10853459
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG335690.30929310185812653TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG50800.037.25346832
CGTCCGC51150.036.60396633
GTCCGCA53150.035.35343634
TCCGCAC53850.034.80949435
AGTCACG55300.034.6652928
CACGTCC54700.034.59657331
GTCACGT57000.033.4360529
TCGTATG58100.033.08569345
ACACGTC61000.032.97023413
CGCACAT57850.032.75058737
GCACACG61850.032.44437811
ACGTCTG62000.032.4024715
CTCGTAT59150.032.3842144
CACGTCT62750.031.9793414
CACACGT63200.031.8581312
TCACGTC59900.031.81830230
CCGCACA59900.031.2181136
CGTCTGA64500.031.21531316
CAGTCAC61750.030.89749327
CCAGTCA63200.030.75650826
Produced by FastQC (version 0.11.2)