FastQCFastQC Report
Wed 4 Feb 2015
SE352_GTAGAG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE352_GTAGAG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10236006
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC478550.46751633400761977TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63400.035.67844444
CGTAGAG63300.035.44255433
AGTCACG64750.035.27028328
GTATGCC65150.034.75462745
ACGTCTG68550.034.19714715
CACGTAG66750.033.91227731
TCGTATG67050.033.8369243
GCACACG70300.033.53787611
TCACGTA67450.033.5276330
CTCGTAT67600.033.4596142
ACACGTC70150.033.41716813
GTCACGT67500.033.4014829
CACGTCT70450.033.1792314
CACACGT71450.032.9351112
CAGTCAC70300.032.6131727
CGTCTGA72600.032.25736216
GATCTCG70500.032.04569239
CCAGTCA72200.031.75322526
GAACTCC73750.031.63327821
ACGTAGA71750.031.61208332
Produced by FastQC (version 0.11.2)