FastQCFastQC Report
Wed 4 Feb 2015
SE351_CCGTCC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE351_CCGTCC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11496496
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG155600.13534558703799834TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG22400.032.54900435
CCGTCCC24050.030.41014134
CCCGTCC24650.029.48747833
GTCACCC26450.028.58332629
GTCCCGA25250.028.42930436
ACCCGTC26150.028.3968232
TCACCCG26950.027.88761730
CACCCGT26750.027.59080331
CCCGATC26100.027.32910738
ACACGTC32600.024.77413613
AGTCACC30900.024.6842328
ACGTCTG34100.023.75054615
CACGTCT34550.023.11553214
GCACACG34250.022.98948111
CTCGTAT32550.022.61192944
TCGTATG31800.022.57898545
CACACGT35750.022.40245612
CGTCTGA36800.021.94598616
CCGATCT33500.021.42740439
GATCTCG34400.021.26261341
Produced by FastQC (version 0.11.2)