FastQCFastQC Report
Wed 4 Feb 2015
SE350_ATGTCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE350_ATGTCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7236232
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG260860.3604914823073666TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40800.033.79928611
ACACGTC41900.033.01933313
CACGTCT42250.032.79904614
ACGTCTG42400.032.78935215
CAGTCAC41100.032.62681627
CACACGT42700.032.3480212
CTCGTAT41050.031.9146644
TCGTATG41150.031.67299545
ACTCCAG43050.031.56421723
CCAGTCA43350.031.29366526
CGTCTGA44600.030.96975316
TCCAGTC44450.030.36761725
GAACTCC45950.030.20861821
AGTCACA45650.029.27688828
ACATGTC44250.029.24050132
ATGTCAG44450.029.0601234
GATCGGA49300.028.9098131
AGCACAC48750.028.7021210
GTCACAT46900.028.21088629
CTCCAGT48300.028.08674424
Produced by FastQC (version 0.11.2)