FastQCFastQC Report
Wed 4 Feb 2015
SE349_AGTTCC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE349_AGTTCC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10164035
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26250.022.96721815
GCACACG26550.022.70770311
ACACGTC26450.022.53840313
CACGTCT27100.022.24684514
CCGTATC24100.021.28816638
CGTATCT24800.021.14189739
CACACGT28850.020.89738512
GTCACAG27900.019.436829
CGTCTGA30650.019.37626316
GTTCCGT28200.019.07203535
CAGTCAC29300.018.89011227
CTCGTAT27650.018.88606844
TCGTATG28050.018.61674945
CCAGTCA30700.018.39429526
TCCGTAT28550.018.20649337
GAACTCC32850.017.94341921
AGTTCCG29950.017.73223334
GTATCTC29750.017.70085540
ACTCCAG32450.017.4022223
GATCGGA34850.017.2496851
Produced by FastQC (version 0.11.2)