FastQCFastQC Report
Wed 4 Feb 2015
SE348_TTAGGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE348_TTAGGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282693
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC110710.10766634771649801TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG16900.032.48689731
ACTTAGG17550.031.15516732
GCACACG23450.026.57429911
CACACGT24350.025.59171312
CGTATGC23100.024.35843344
ACACGTC26150.023.82956113
TAGGCAT23700.023.45207235
GTATGCC24500.023.4257445
TTAGGCA24350.022.91856634
ACGTCTG27400.022.74222415
CAGTCAC26350.022.54255327
TCGTATG26350.021.43938643
AGGCATC26800.020.82238436
CACGTCT31650.019.68837214
CTCGTAT28950.019.43569442
CGTCTGA33000.019.428216
AGCACAC33150.019.00200310
GGCATCT29650.018.51680637
AGTCACT31350.018.2302628
TCTCGTA31700.017.7485141
Produced by FastQC (version 0.11.2)