FastQCFastQC Report
Wed 4 Feb 2015
SE347_CGATGT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE347_CGATGT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16402850
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG24450.010.85691815
ACACGTC25200.010.53379613
GCACACG24300.09.9982411
CGTATGC18950.09.97745644
CACGTCT26800.09.90491314
CACACGT27700.09.42072412
GTATGCC21100.08.53411845
CGTCTGA31400.08.45387516
CTCGTAT23850.08.3047842
GTCACCG21250.07.941134529
AGTCACC22400.07.935008528
CAGTCAC27600.07.581250727
ACTCCAG33100.07.34108223
TCGTATG26800.07.306895343
CCGATGT24100.07.282674333
GAACTCC36250.07.19943521
CCAGTCA31900.07.05308226
AGCACAC39750.06.734663510
CGATGTA26050.06.651244634
TCCAGTC34500.06.58674125
Produced by FastQC (version 0.11.2)