FastQCFastQC Report
Wed 4 Feb 2015
SE346_GGTAGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE346_GGTAGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24411962
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC3055941.2518207262488776TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG367050.041.81752429
CACGGTA358500.041.61783231
GGTAGCA353950.041.44981834
GCACACG383850.041.42741411
CGGTAGC356200.041.29404433
ACGTCTG385300.041.20144715
ACACGTC387250.041.10434713
AGTCACG374500.041.08593828
CACACGT389850.040.8302112
CGTATGC375450.040.7997244
CACGTCT390100.040.73501214
GTATGCC378500.040.52109545
TCACGGT369200.040.4487530
GTAGCAT363350.040.39945635
CGTCTGA392650.040.08637216
CTCGTAT384150.040.01591542
TAGCATC368700.039.84057636
TCGTATG386800.039.75954443
CAGTCAC392700.039.46342527
AGCACAC406500.039.2795810
Produced by FastQC (version 0.11.2)