FastQCFastQC Report
Wed 4 Feb 2015
SE345_GAGTGG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE345_GAGTGG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11939873
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC385520.32288450639299093TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC59650.034.4692113
CACACGT60250.034.05098712
ACGAGTG55100.033.85665532
GCACACG61100.033.68773711
CACGTCT62550.032.94304314
CTCGTAT59450.032.8606642
CGAGTGG56250.032.72564733
AGTCACG58800.032.67928328
CACGAGT57550.032.25796531
CGTATGC60900.032.18953744
TCGTATG61000.032.09987343
GTATGCC62950.031.28664245
GTCACGA61650.030.73149729
GAACTCC68100.030.19497521
ACGTCTG68800.029.98309715
AGCACAC69700.029.91843610
CAGTCAC65100.029.8601527
TCACGAG63200.029.83738530
GATCTCG64800.029.58670439
GGATCTC64750.029.26226638
Produced by FastQC (version 0.11.2)