FastQCFastQC Report
Wed 4 Feb 2015
SE344_CGTACG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE344_CGTACG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579542
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC536210.6249867417165158TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG62550.040.43975433
CGTACGA63850.039.40606334
GCACACG75700.038.9573111
ACACGTC76650.038.38665813
CACGTCT77500.037.93683614
ACGTCTG77650.037.7474515
CACACGT78050.037.72670712
CACCGTA67950.037.72102431
GTATGCC73850.037.643745
GTCACCG70000.037.6435329
AGTCACC71200.037.38616628
CGTATGC74450.037.27746644
GTACGAT68450.036.98738535
CGTCTGA79800.036.75820516
TACGATC69700.036.41794636
CTCGTAT76350.036.34937742
CAGTCAC77650.036.18984227
TCGTATG77250.036.07243
CCAGTCA79650.035.90256526
TCACCGT72050.035.7941930
Produced by FastQC (version 0.11.2)