FastQCFastQC Report
Wed 4 Feb 2015
SE343_AGTCAA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE343_AGTCAA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8231187
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC6862328.336974970924608TruSeq Adapter, Index 13 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG760300.043.80671711
CACGTCT758100.043.78573214
ACACGTC759150.043.78120813
CACACGT760000.043.7707212
GATCGGA769000.043.697741
ACGTCTG758950.043.67444615
CGTCTGA759900.043.6010216
AGCACAC765800.043.57969310
TCGGAAG773300.043.5703853
AGAGCAC771750.043.3796588
CGGAAGA776550.043.3171964
GTATGCC751500.043.11275545
ATCGGAA780500.043.0835572
GAGCACA776000.043.067759
GAACTCC765800.043.03832621
GAAGAGC780150.043.019276
TCCAGTC763600.042.904125
CAGTCAC762500.042.9031627
CCAGTCA762800.042.89626
GTCACAG761450.042.86602829
Produced by FastQC (version 0.11.2)