FastQCFastQC Report
Wed 4 Feb 2015
SE342_GCCAAT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE342_GCCAAT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13499629
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC9906847.338601675646049TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1147700.043.99125313
CACGTCT1144750.043.9849114
GCACACG1152250.043.9442811
CACACGT1153400.043.86925512
ACGTCTG1148550.043.82159815
CGTCTGA1148550.043.79417816
AGAGCAC1162850.043.768278
GAGCACA1164850.043.6581959
AGCACAC1162350.043.62823510
TCGGAAG1163200.043.5968443
GAACTCC1138100.043.5508421
GTATGCC1141300.043.5270945
GATCGGA1165300.043.4808431
CGGAAGA1170400.043.43824
AGTCACG1136500.043.33661328
TCTGAAC1156350.043.31022618
CTCCAGT1137000.043.3038124
GAAGAGC1178850.043.3019146
CTCGTAT1144700.043.24664742
CTGAACT1158850.043.19770419
Produced by FastQC (version 0.11.2)