FastQCFastQC Report
Wed 4 Feb 2015
SE341_ACAGTG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE341_ACAGTG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10182320
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC114931611.287368693971512TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1331400.044.08388512
ACACGTC1330700.044.07833513
GCACACG1332700.044.0780211
ACGTCTG1328200.044.02220515
CACGTCT1331450.043.98085814
CGTCTGA1332000.043.84382616
AGCACAC1342100.043.83131410
GTATGCC1321050.043.7660445
GAACTCC1320650.043.66410421
AGAGCAC1354000.043.596858
GTCACAC1309750.043.55850629
GAGCACA1356300.043.5034459
CTGAACT1336150.043.4957319
ACTCCAG1316850.043.47616623
CTCCAGT1315600.043.42534324
CAGTCAC1317400.043.4104127
CTCGTAT1326950.043.39819342
TCCAGTC1316150.043.39180825
TGAACTC1337000.043.3865120
TCTGAAC1340650.043.37972618
Produced by FastQC (version 0.11.2)