FastQCFastQC Report
Wed 4 Feb 2015
SE340_TGACCA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE340_TGACCA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14229397
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC299456121.044890377294273TruSeq Adapter, Index 4 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAACAATCTCGTATGCC208030.1461973406181583TruSeq Adapter, Index 4 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTAATCTCGTATGCC143180.100622675718444TruSeq Adapter, Index 4 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3436650.044.4899713
CACACGT3444700.044.47807712
GCACACG3448800.044.47607811
CACGTCT3438150.044.44127314
ACGTCTG3441900.044.3882815
AGCACAC3461650.044.34866710
CGTCTGA3447450.044.28549216
AGAGCAC3494300.044.258598
GAGCACA3492400.044.2068189
TCGGAAG3498550.044.17623
GATCGGA3497950.044.13911
CGGAAGA3510500.044.1321954
TCTGAAC3457350.044.1176918
GAACTCC3436700.044.09861821
GAAGAGC3519300.044.098246
CTGAACT3459500.044.0832819
AAGAGCA3521300.044.0687187
GTATGCC3422250.044.0300645
GTCTGAA3481350.044.00481817
TGAACTC3463600.043.99688720
Produced by FastQC (version 0.11.2)