FastQCFastQC Report
Wed 4 Feb 2015
SE339_TTAGGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE339_TTAGGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10840451
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC109505410.101553892914602TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG1177650.044.54586432
CACTTAG1181500.044.53928431
GTATGCC1203900.044.431645
CACGTCT1214350.044.34491714
ACACGTC1214600.044.32817513
ACGTCTG1216250.044.32147215
CGTATGC1208750.044.24839844
TTAGGCA1172650.044.22293534
CGTCTGA1218300.044.215516
AGGCATC1171150.044.21278836
CACACGT1219600.044.18886612
GAACTCC1216000.044.18028321
GGCATCT1173700.044.17766637
TCGTATG1211950.044.17737643
CTCGTAT1207200.044.1703742
ACTCCAG1211900.044.13355323
GCACACG1222250.044.11882811
CCAGTCA1210150.044.06618526
CAGTCAC1210450.044.062727
TCCAGTC1211150.044.0539525
Produced by FastQC (version 0.11.2)