FastQCFastQC Report
Wed 4 Feb 2015
SE337_ATCACG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE337_ATCACG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33038431
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC365623711.066618145395585TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4140750.044.02156413
ACGTCTG4127000.044.01391615
CACGTCT4138950.043.9728414
CACACGT4150250.043.95277812
CGTCTGA4134900.043.89915516
GCACACG4164350.043.86337711
GTATGCC4078550.043.84449845
GAACTCC4121800.043.71931521
CTGAACT4154600.043.5420919
ACTCCAG4112450.043.5249823
AGCACAC4205750.043.52038610
TCTGAAC4162250.043.5182818
TGAACTC4156850.043.49452220
CTCGTAT4107600.043.48649642
TCCAGTC4110950.043.4745325
CTCCAGT4108100.043.46799524
CGTATGC4116800.043.4477344
ACGATCT4045450.043.4437
TCGTATG4127350.043.40688743
CACGATC4025200.043.39401236
Produced by FastQC (version 0.11.2)