FastQCFastQC Report
Wed 4 Feb 2015
SE336_GTCCGC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE336_GTCCGC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3905060
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG1211043.101207151746708TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG152450.043.4703311
CGTCCGC143600.043.37932633
ACGTCCG145150.043.31643732
GTCCGCA143650.043.27355634
AGTCACG150300.043.2700928
CACGTCC145550.043.15266831
GATCGGA154350.043.129561
ACACGTC153050.043.12353513
CGTCTGA153200.043.1084716
CACACGT153900.043.0315312
TCACGTC147750.042.99972530
AGCACAC154650.042.9246610
ACGTCTG154250.042.8739115
TCCGCAC144800.042.85051335
GTCACGT149000.042.832629
CACGTCT154050.042.82900614
CGCACAT147150.042.6949237
GAACTCC153450.042.66148421
ACTCCAG152550.042.6215123
CAGTCAC153300.042.6200927