Basic Statistics
Measure | Value |
---|---|
Filename | SE336_GTCCGC_L004_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3905060 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 36 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG | 121104 | 3.101207151746708 | TruSeq Adapter, Index 18 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 15245 | 0.0 | 43.47033 | 11 |
CGTCCGC | 14360 | 0.0 | 43.379326 | 33 |
ACGTCCG | 14515 | 0.0 | 43.316437 | 32 |
GTCCGCA | 14365 | 0.0 | 43.273556 | 34 |
AGTCACG | 15030 | 0.0 | 43.27009 | 28 |
CACGTCC | 14555 | 0.0 | 43.152668 | 31 |
GATCGGA | 15435 | 0.0 | 43.12956 | 1 |
ACACGTC | 15305 | 0.0 | 43.123535 | 13 |
CGTCTGA | 15320 | 0.0 | 43.10847 | 16 |
CACACGT | 15390 | 0.0 | 43.03153 | 12 |
TCACGTC | 14775 | 0.0 | 42.999725 | 30 |
AGCACAC | 15465 | 0.0 | 42.92466 | 10 |
ACGTCTG | 15425 | 0.0 | 42.87391 | 15 |
TCCGCAC | 14480 | 0.0 | 42.850513 | 35 |
GTCACGT | 14900 | 0.0 | 42.8326 | 29 |
CACGTCT | 15405 | 0.0 | 42.829006 | 14 |
CGCACAT | 14715 | 0.0 | 42.69492 | 37 |
GAACTCC | 15345 | 0.0 | 42.661484 | 21 |
ACTCCAG | 15255 | 0.0 | 42.62151 | 23 |
CAGTCAC | 15330 | 0.0 | 42.62009 | 27 |