FastQCFastQC Report
Wed 4 Feb 2015
SE335_GTAGAG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE335_GTAGAG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3829888
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC173460.45291141673072427TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25200.038.03016311
AGTCACG22550.038.0184128
ACGTCTG25550.037.33310315
ACACGTC25750.037.0436213
CGTAGAG21250.036.5336233
GTATGCC24850.036.3186745
GTCACGT22250.036.20594429
CGTATGC25150.035.8835744
CACGTCT26600.035.85989814
CTCGTAT25500.035.47884442
CACACGT27100.035.36430412
TCACGTA22600.035.14831530
CGTCTGA27050.034.929316
CACGTAG23050.034.75531
TCGTATG26300.034.4852143
CAGTCAC26100.033.79214527
CCAGTCA27400.033.4205826
GATCTCG25950.032.94962339
AGCACAC29350.032.80611810
TCCAGTC28600.032.25432625
Produced by FastQC (version 0.11.2)