FastQCFastQC Report
Wed 4 Feb 2015
SE334_CCGTCC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE334_CCGTCC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469861
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1460795.914462392822916TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG174650.044.06416311
ACGTCTG174500.044.04958315
CACGTCT174350.044.03676214
ACACGTC174700.044.0129213
CCCGTCC165400.043.97659333
CCGTCCC163850.043.95307534
CGTCCCG163250.043.92161635
CACCCGT167500.043.9188231
TCACCCG169300.043.87544330
AGCACAC175750.043.85236710
CACACGT175250.043.84911712
GTCCCGA160200.043.8402536
ACCCGTC167850.043.8138332
GTCACCC171250.043.7918929
GATCGGA177150.043.705971
CCCGATC161250.043.68037438
AGTCACC172300.043.63901528
CAGTCAC173650.043.5803827
CGTCTGA176500.043.54955316
TCGTATG165750.043.4216945