FastQCFastQC Report
Wed 4 Feb 2015
SE333_ATGTCA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE333_ATGTCA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888563
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG296830.7633411108422314TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40600.039.39285711
ACACGTC41100.039.18728313
CACACGT41300.038.77964812
ACGTCTG41950.038.6623615
CACGTCT42150.038.21206714
CGTCTGA42900.037.91058716
CAGTCAC41750.037.66608427
TCCAGTC43300.036.8888125
GAACTCC44150.036.68627521
ACTCCAG43800.036.67315723
CCAGTCA43600.036.4806626
AGCACAC44550.036.10208510
TCGTATG41200.036.0060145
CTCGTAT41050.035.91591644
CTCCAGT44550.035.75277324
GATCGGA46050.035.7052041
ACATGTC41650.035.50112532
ATGTCAG42100.035.28384834
AGTCACA44450.035.13051628
AGAGCAC47100.034.3863078
Produced by FastQC (version 0.11.2)