FastQCFastQC Report
Wed 4 Feb 2015
SE332_AGTCAA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE332_AGTCAA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241627
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC1312283.0938128222967274TruSeq Adapter, Index 13 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG162750.042.90421311
CACGTCT162550.042.84628714
ACACGTC162900.042.7818513
CACACGT163050.042.7700912
ACGTCTG162850.042.5872815
GATCGGA164900.042.4722331
CGTCTGA164750.042.10978716
AGCACAC166850.041.89037710
CACAGTC153700.041.8008531
GAACTCC164800.041.7889721
GTATGCC161200.041.778445
TCGGAAG167900.041.7087943
GTCACAG158250.041.66502829
CAGTCAC162400.041.6044927
CGTATGC161850.041.59376544
TCCAGTC163800.041.59229325
CCAGTCA163800.041.55108626
ACAGTCA154700.041.5000832
CTCGTAT162650.041.47025742
TCGTATG163000.041.45073743
Produced by FastQC (version 0.11.2)