FastQCFastQC Report
Wed 4 Feb 2015
SE331_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE331_CTTGTA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7691611
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2824893.672689635500287TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC329300.043.1733913
ACGTCTG329900.042.98520715
CACACGT331600.042.9349912
CACGTCT330950.042.92415214
GCACACG332450.042.91994511
AGTCACC322800.042.87198328
GTATGCC326750.042.73504645
GTCACCT322000.042.70789329
CGTATGC327400.042.62625544
CCTTGTA318750.042.61634433
CGTCTGA333400.042.5539216
CTCGTAT327400.042.5225942
CACCTTG322300.042.48798431
ACCTTGT321400.042.4179132
TCGTATG329900.042.37827343
GAACTCC333850.042.2996421
TCCAGTC331450.042.26388525
CAGTCAC331200.042.26181827
TGTAATC321650.042.23334536
CCAGTCA332050.042.1739626