FastQCFastQC Report
Wed 4 Feb 2015
SE329_GATCAG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE329_GATCAG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773586
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC5548528.191407033143154TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT629850.044.09454312
ACACGTC629900.044.0517613
GCACACG633100.043.9392511
CACGTCT631100.043.92878714
AGCACAC635650.043.79483410
TCGGAAG637650.043.7386133
AGAGCAC639300.043.7239278
GATCGGA637150.043.6861571
GAGCACA641150.043.555989
GTATGCC628350.043.55315845
ACGTCTG636650.043.53909715
CTCGTAT627300.043.45876742
GAACTCC632000.043.45783221
CGTCTGA639300.043.34070216
TCTCGTA628650.043.3176241
ATCGGAA644000.043.2581332
AGTCACG626150.043.2456928
TCGTATG633950.043.22300343
AGATCTC620550.043.2066838
GATCTCG625050.043.2013139
Produced by FastQC (version 0.11.2)