FastQCFastQC Report
Wed 4 Feb 2015
SE328_ACTTGA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE328_ACTTGA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9594875
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC6024546.278914524681145TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC695700.043.9792713
CACACGT697950.043.8858412
CACGTCT698450.043.75480314
GCACACG701150.043.73046511
GAACTCC698800.043.560621
GTCACAC681150.043.51925329
GTATGCC693200.043.4068645
CTCGTAT693150.043.38756642
ACGTCTG703850.043.35838715
AGCACAC709300.043.31679510
CACTTGA674750.043.2363733
TCGTATG697800.043.20833243
TCTCGTA696150.043.19510341
TCACACT683550.043.10114330
ACACTTG680300.043.09001532
ACTCCAG702600.043.08686423
AGAGCAC716000.043.080228
CTCCAGT700750.043.0792824
GAGCACA716650.043.0137949
CGTATGC699650.043.01066244
Produced by FastQC (version 0.11.2)