FastQCFastQC Report
Wed 4 Feb 2015
SE327_CAGATC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE327_CAGATC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8530359
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2902063.4020373585683794TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT328300.043.0072442
CACGTCT333850.042.9889614
ACACGTC335850.042.8267313
TCGTATG331250.042.7261643
CACACGT336700.042.6918912
TCTCGTA331600.042.63004341
GTATGCC332050.042.55817845
CGTATGC332650.042.47892444
GAACTCC336400.042.46027421
CATCTCG325550.042.44819639
GCACACG339700.042.40094811
ACTCCAG338850.041.92286323
CTCCAGT337600.041.9124624
ACGTCTG343050.041.901415
AGATCAT327900.041.7273135
TCTGAAC343700.041.704118
ATCTCGT339800.041.66362840
TCCAGTC339350.041.66317425
TGAACTC344150.041.626620
AGCACAC346100.041.61688210
Produced by FastQC (version 0.11.2)