FastQCFastQC Report
Wed 4 Feb 2015
MK249_AGTTCC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK249_AGTTCC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11340148
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3196022.818323006013678TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG409400.043.10993615
CACACGT413250.042.83369412
GTCACAG408550.042.7521729
CGTATCT405150.042.46970739
CACGTCT415000.042.4685914
AGTCACA412950.042.40302328
GCACACG417650.042.39264711
CCGTATC405550.042.3338638
CGTCTGA416600.042.30008316
TATCTCG411500.042.12114741
CAGTCAC417000.041.9692727
TCGTATG410950.041.94478645
TCACAGT415750.041.88186630
GTATCTC413650.041.7977540
GTCTGAA423300.041.75809517
ACACGTC426500.041.5608213
AGCACAC427100.041.5391210
CACAGTT419800.041.51575531
CTCGTAT415900.041.43221744
TCCGTAT417350.041.34721837
Produced by FastQC (version 0.11.2)