FastQCFastQC Report
Wed 4 Feb 2015
MK248_CTTGTA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK248_CTTGTA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9573453
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC17250.020.87245428
GTCACCT18000.018.62767429
ACGTCTG22550.017.45732115
GTATGCC20400.017.21482845
CACACGT24650.016.51763212
CAGTCAC22450.016.33702727
CGTCTGA24800.016.14560916
CACGTCT26400.015.59312714
CGTATGC22850.015.36454244
GCACACG27900.014.59338111
GTCTGAA28300.013.98982617
AGCACAC33500.012.82544310
TCGTATG28050.012.75684343
CTCGTAT28350.012.62184942
ACACGTC34050.012.28801913
CCGGGAT11350.012.1035431
CGGGATT25500.012.0109481
CCCGGGT6200.011.9867331
ACCTTGT30050.011.75715732
CCCGGTT12950.011.6515471
Produced by FastQC (version 0.11.2)