FastQCFastQC Report
Wed 4 Feb 2015
MK247_GGCTAC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK247_GGCTAC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19161063
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC396520.2069405022049142TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA59600.036.1732732
CGGCTAC59150.035.9555633
GCTACAT59250.035.89280735
GGCTACA60550.035.23608834
CTACATC62050.034.20034836
CACGGCT70750.030.72460731
GCACACG81150.028.7728211
CGTCTGA81400.028.43618616
CACACGT82200.027.94065312
CGTATGC78600.027.80341544
TACATCT78250.027.32120937
GTATGCC80250.027.2390145
TCACGGC82050.026.5510230
ACGTCTG87250.026.50379415
GTCTGAA91250.025.76106517
CAGTCAC91650.024.45171227
CACGTCT94600.024.44456914
CTCGTAT93050.023.87142842
CTGAACT101750.023.05851719
AGCACAC106500.022.55778110
Produced by FastQC (version 0.11.2)