FastQCFastQC Report
Wed 4 Feb 2015
MK246_TAGCTT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK246_TAGCTT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17700647
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1217800.6879974500367134TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTTA150800.042.81764234
ACTAGCT155750.041.5544532
CACTAGC158400.040.8142131
TCACTAG161250.040.24069630
GTCACTA161500.040.23130429
TATCTCG167800.038.878739
CTAGCTT167750.038.6371533
GCACACG174400.038.2809511
CACACGT175600.037.8663812
CGTCTGA175750.037.73156716
CGTATGC173100.037.6900144
CTTATCT172550.037.62585437
GTATGCC173700.037.5327345
AGCTTAT172950.037.40871435
AGTCACT176300.037.14632428
ACGTCTG180750.036.81226315
TTATCTC180150.036.25088538
CACGTCT186350.035.64566414
CTCGTAT185050.035.43731742
GTCTGAA188100.035.38578417