FastQCFastQC Report
Wed 4 Feb 2015
MK245_GATCAG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK245_GATCAG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8366347
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1448921.7318430612548106TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT192650.042.28176512
CGTCTGA191550.042.18400216
ACGTCTG193900.041.9047715
GCACACG195600.041.81583811
GTATGCC194400.041.18836645
CACGTCT197500.041.07259814
GTCTGAA197900.040.95548217
CGTATGC196700.040.7527644
AGCACAC201650.040.62843310
CAGTCAC198100.040.6172427
AGTCACG199350.040.3438428
CTCGTAT200350.039.996742
TCTGAAC202350.039.94363418
ACACGTC204650.039.7585313
GTCACGA202050.039.66136629
TCGTATG203000.039.63119543
CTGAACT204150.039.55792219
GATCGGA184350.039.541471
CCAGTCA204500.039.379126
GATCAGA201500.039.2679934
Produced by FastQC (version 0.11.2)