FastQCFastQC Report
Wed 4 Feb 2015
MK244_ACTTGA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK244_ACTTGA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111505
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1258380.7810443530880572TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC173250.040.22940429
GCACACG184800.038.74456411
GTATGCC179350.038.68552845
CGTCTGA183250.038.6430116
CACACGT185000.038.52112612
AGTCACA182300.038.2917428
CGTATGC182050.038.19907844
TCACACT187750.037.24569330
ACGTCTG191750.037.0121415
GTCTGAA194100.036.61038217
CTCGTAT192100.036.35090342
ACACTTG192350.036.26833332
CAGTCAC193650.036.2058527
CACGTCT196900.036.05549214
TCGTATG194800.035.89383343
CACACTT198950.035.3692531
AGCACAC204600.035.17110
CTGAACT202000.035.1682219
TCTGAAC206650.034.3761218
CCAGTCA205550.034.2520726
Produced by FastQC (version 0.11.2)