FastQCFastQC Report
Wed 4 Feb 2015
MK243_CAGATC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK243_CAGATC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9247369
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC282180.3051462529504338TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC48050.033.25051528
CACACGT50350.032.9281712
ACGTCTG52000.031.71011515
GTATGCC50200.031.61564645
TCGTATG51200.031.47125643
CGTCTGA52350.031.45513716
CACGTCT53200.030.9102814
CAGTCAC52000.030.8529127
CTCGTAT53350.030.1181142
GTCTGAA55450.029.85887517
CGTATGC54050.029.47919344
ACACGTC59100.027.9006113
GCACACG60900.027.44475211
TCTGAAC60950.027.20138218
TCTCGTA59300.027.17145541
AGCACAC63450.026.6608510
ACCAGAT60550.026.42412632
CCAGTCA61600.026.19077726
TCACCAG60600.026.18107630
CTGAACT64400.025.70909519
Produced by FastQC (version 0.11.2)