FastQCFastQC Report
Wed 4 Feb 2015
MK242_GGTAGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK242_GGTAGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19582970
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC19018439.711718906784823TruSeq Adapter, Index 22 (97% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC638350.3259720052678424TruSeq Adapter, Index 11 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2388650.044.32069412
GCACACG2390900.044.29498311
AGCACAC2406100.044.07686610
ACGTCTG2400950.044.07583615
CGTCTGA2377500.044.07058316
CACGTCT2403750.044.03198214
GATCGGA2222150.043.9884221
GTCTGAA2383850.043.90600217
ACACGTC2416450.043.8229113
CTGAACT2389500.043.74038719
TCTGAAC2394400.043.6818
AGAGCAC2433900.043.634648
ATCGGAA2250100.043.6087232
TCGGAAG2258900.043.5354423
CAGTCAC2402050.043.4660227
GAGCACA2444350.043.449029
GTATGCC2321200.043.44470245
GGTAGCA2273650.043.44350434
GTAGCAT2272450.043.4085735
CGGAAGA2315450.043.3357474
Produced by FastQC (version 0.11.2)