FastQCFastQC Report
Wed 4 Feb 2015
MK241_GAGTGG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK241_GAGTGG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16246043
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC7446194.5833868591878035TruSeq Adapter, Index 7 (97% over 36bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC238000.14649721165947918TruSeq Adapter, Index 9 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT944700.043.55221612
GCACACG952600.043.20520411
CGTCTGA953650.043.15055516
ACGTCTG955000.043.10133715
CACGTCT957650.042.97031814
AGCACAC960800.042.91607310
GTCTGAA960150.042.84685517
CAGTCAC946750.042.81395327
GTATGCC918850.042.7635745
CGTATGC923800.042.5624944
TCTGAAC965700.042.53783818
CTGAACT966550.042.4656519
ACACGTC971800.042.3562213
ACGAGTG917600.042.32250232
GTCACGA947250.042.2760429
CTCGTAT932000.042.2563542
CACGAGT922200.042.2250331
GATCGGA905000.042.1313931
TCGTATG936000.042.1276943
TCACGAG948650.042.11601630