Basic Statistics
Measure | Value |
---|---|
Filename | MK241_GAGTGG_L002_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16246043 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 51 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC | 744619 | 4.5833868591878035 | TruSeq Adapter, Index 7 (97% over 36bp) |
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC | 23800 | 0.14649721165947918 | TruSeq Adapter, Index 9 (97% over 35bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACACGT | 94470 | 0.0 | 43.552216 | 12 |
GCACACG | 95260 | 0.0 | 43.205204 | 11 |
CGTCTGA | 95365 | 0.0 | 43.150555 | 16 |
ACGTCTG | 95500 | 0.0 | 43.101337 | 15 |
CACGTCT | 95765 | 0.0 | 42.970318 | 14 |
AGCACAC | 96080 | 0.0 | 42.916073 | 10 |
GTCTGAA | 96015 | 0.0 | 42.846855 | 17 |
CAGTCAC | 94675 | 0.0 | 42.813953 | 27 |
GTATGCC | 91885 | 0.0 | 42.76357 | 45 |
CGTATGC | 92380 | 0.0 | 42.56249 | 44 |
TCTGAAC | 96570 | 0.0 | 42.537838 | 18 |
CTGAACT | 96655 | 0.0 | 42.46565 | 19 |
ACACGTC | 97180 | 0.0 | 42.35622 | 13 |
ACGAGTG | 91760 | 0.0 | 42.322502 | 32 |
GTCACGA | 94725 | 0.0 | 42.27604 | 29 |
CTCGTAT | 93200 | 0.0 | 42.25635 | 42 |
CACGAGT | 92220 | 0.0 | 42.22503 | 31 |
GATCGGA | 90500 | 0.0 | 42.131393 | 1 |
TCGTATG | 93600 | 0.0 | 42.12769 | 43 |
TCACGAG | 94865 | 0.0 | 42.116016 | 30 |