FastQCFastQC Report
Wed 4 Feb 2015
MK240_CGTACG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK240_CGTACG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12747726
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC9263867.267068652087438TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1171100.044.0403412
GCACACG1175850.043.9121811
GATCGGA1086800.043.844681
CGTCTGA1175900.043.78995516
ACGTCTG1178050.043.7444115
AGCACAC1184400.043.6787610
ACCGTAC1121650.043.6500632
CCGTACG1114450.043.64183833
CACGTCT1180550.043.64034314
ATCGGAA1100200.043.4805342
CGTACGA1110050.043.4173534
TCGGAAG1102700.043.3957253
GTCTGAA1185950.043.39248317
CACCGTA1135050.043.38780631
AGTCACC1150600.043.35383628
TCTGAAC1187250.043.31671518
AGAGCAC1198200.043.283068
ACACGTC1191600.043.2675813
GTACGAT1119450.043.23953635
CTGAACT1190400.043.18353319
Produced by FastQC (version 0.11.2)