FastQCFastQC Report
Wed 4 Feb 2015
MK239_CCGTCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK239_CCGTCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15966141
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG185460411.615856329967272TruSeq Adapter, Index 16 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG641330.4016812829098778TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTATG361430.22637279728395235TruSeq Adapter, Index 16 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2506950.044.3986112
GCACACG2507600.044.39158611
ACGTCTG2505600.044.38240415
CGTCTGA2512150.044.26131416
CACGTCT2512300.044.2380714
AGCACAC2523300.044.20809610
CACCCGT2399500.044.16720231
GTCACCC2443700.044.1567129
TCACCCG2434350.044.1417830
CCGTCCC2339300.044.13677634
GTCTGAA2520900.044.12463417
ACCCGTC2394950.044.1076732
GATCGGA2329800.044.1076351
CCCGTCC2375000.044.07131633
CGTCCCG2333250.044.06453735
AGTCACC2470850.044.0025228
CTGAACT2526700.043.9678119
GTCCCGA2263000.043.96677436
ACACGTC2530700.043.95171413
TCTGAAC2532400.043.8773218
Produced by FastQC (version 0.11.2)