FastQCFastQC Report
Wed 4 Feb 2015
MK238_GTGGCC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK238_GTGGCC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27301851
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC16332975.9823672761235125TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2065750.043.88620411
CACACGT2063850.043.88300712
CGTCTGA2066450.043.7257816
AGCACAC2085300.043.5427310
ACGTCTG2078300.043.52409415
CGTGGCC1930050.043.49799333
CACGTCT2081650.043.4713414
GTCTGAA2084950.043.3011117
GTATGCC1986950.043.1942945
CTGAACT2089450.043.14687719
GATCGGA1943600.043.130651
TCTGAAC2095150.043.05594618
CAGTCAC2073500.043.0082327
GTGGCCA1942900.043.00044634
ACACGTC2112050.042.87202513
TCACGTG2015750.042.8260930
CGTATGC2006100.042.80353544
GTCACGT2030050.042.78281829
GCCATCT1971700.042.64336837
AGAGCAC2135250.042.613848