FastQCFastQC Report
Wed 4 Feb 2015
MK237_GTGAAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK237_GTGAAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473451
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG107794210.29213771086531TruSeq Adapter, Index 19 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG356910.34077592953841096TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATG155940.14889075243680425TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATTTCGTATG114950.10975370009369403TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1410600.044.3781112
ACGTCTG1401800.044.3662315
GCACACG1412450.044.33909211
CGTCTGA1403800.044.25174316
CACGTCT1405900.044.23524514
ACACGTC1408350.044.21579713
AGCACAC1424150.044.08698310
GTCTGAA1418350.043.95480717
GATCGGA1327850.043.897331
TCTGAAC1416150.043.7802618
CACGTGA1371800.043.75601631
CGGAAGA1369600.043.7434164
TCGTATG1356250.043.7428545
GTCACGT1383750.043.7313429
AGAGCAC1443950.043.7286038
CGTGAAA1362850.043.7154333
ACGTGAA1372850.043.67215732
CTGAACT1416650.043.6647619
TCACGTG1381500.043.63279730
CAGTCAC1410750.043.62684227
Produced by FastQC (version 0.11.2)