FastQCFastQC Report
Wed 4 Feb 2015
MK236_GTCCGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK236_GTCCGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9449847
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG7553667.993420422574038TruSeq Adapter, Index 18 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG289480.3063330020052176TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1016950.044.0552412
GCACACG1018750.043.9707811
CGTCTGA1020700.043.8801616
AGCACAC1025200.043.79288510
CCGCACA948850.043.79144336
CGCACAT958600.043.74106237
GTCTGAA1026900.043.72915317
ACGTCTG1025500.043.72303815
ACACGTC1024900.043.6718113
TCGTATG985000.043.583345
CACGTCT1027550.043.53509514
CAGTCAC1020250.043.51926427
GATCGGA953900.043.5187871
CGTCCGC963500.043.47331633
GTCACGT1000400.043.46575529
TCCGCAC953400.043.42196735
CTGAACT1024650.043.40825319
GTCCGCA963350.043.3446234
TCACGTC991050.043.32845730
TCTGAAC1027650.043.3231218
Produced by FastQC (version 0.11.2)