FastQCFastQC Report
Wed 4 Feb 2015
MK235_GTAGAG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK235_GTAGAG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6923833
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC89586112.938801383568899TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1115150.044.21917712
GATCGGA1041050.044.1466141
GCACACG1119450.044.09554311
AGCACAC1125300.043.98625610
ACGTCTG1119750.043.96319215
CACGTCT1121150.043.93036314
ACACGTC1122650.043.87367213
CGGAAGA1075250.043.8323634
CGTCTGA1122050.043.82495516
AGAGCAC1133400.043.806878
TCGGAAG1056600.043.776583
ATCGGAA1055200.043.7740942
GTATGCC1085850.043.7461645
GTCTGAA1124700.043.67569417
GAGCACA1137600.043.57799
CTGAACT1129000.043.4083519
TCACGTA1082850.043.37713230
TCTGAAC1130900.043.3752118
CAGTCAC1121750.043.30716727
CACGTAG1083650.043.25977731
Produced by FastQC (version 0.11.2)